Table 2. Information about the antibodies used in this study.
| Antibody | Name | Provider | Method | Dilution |
|---|---|---|---|---|
| P-ERK1/2 | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody | Cell Signaling | WB | 1/2000 |
| ERK1/2 | p44/42 MAPK (Erk1/2) Antibody | Cell Signaling | WB | 1/1000 |
| TPL2 | Cot (M-20) | Santa Cruz Biotechnology, Inc | WB | 1/1000 |
| ABIN2 | ABIN2 antibody | S.C. Ley laboratory | WB | 2 µg/ml |
| P-p38 | Phospho-p38 MAP Kinase (Thr180/Tyr182) Antibody | Cell Signaling | WB | 1/1000 |
| p38α | p38α (C-20) | Santa Cruz Biotechnology, Inc | WB | 1/1000 |
| p38γ | p38γ (GST-SAPK3) S524A 1st Bleed | DSTT* | WB | 1/1000 |
| p38δ | p38δ (GST-SAPK4) S526A 3rd Bleed | DSTT* | WB | 1/1000 |
| P-JNK1/2 | Rabbit (polyclonal) Anti-JNK1&2 [pTpY183/185] Phosphospecific Antibody, Unconjugated | Biosource | WB | 1/1000 |
| JNK1/2 | SAPK/JNK | Cell Signaling | WB | 1/1000 |
| P-p105 | Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb | Cell Signaling | WB | 1/1000 |
| p105 | NF-κB1 p105 Antibody | Cell Signaling | WB | 1/1000 |
| hDlg | hDlg (SAP97) antibody | DSTT* | WB | 1/1000 |
| P-hDlg (S158) | Phospho-hDlg (SAP97) Ser158 | DSTT* | WB | 1/1000 |
| hDlg | hDlg (SAP97) antibody | DSTT* | IP | 1 μg/IP |
| p38γ | p38γ (SAPK3 C-Terminal) [KPPRNLGARVPKETA] | DSTT* | IP | 2 μg/IP |
| CD45 | CD45 Monoclonal Antibody (30-F11), APC #17-0451-82 | eBioscience | FAC | 1/100 |
| F4/80 | F4/80 Monoclonal Antibody (BM8) FITC #11-4801-85 | eBioscience | FAC | 1/100 |
| Ly6G | PE Rat Anti-Mouse Ly-6G Clone 1A8 (RUO) #551461 | BD Bioscience | FAC | 1/50 |
*
DSTT (Dundee): Division of Signal Transduction Therapy; University of Dundee (Dundee, UK).