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. 2023 May 22;34(8):1381–1397. doi: 10.1681/ASN.0000000000000154

Figure 3.

Figure 3

miR-147 is increased in cold storage-associated transplantation kidneys and human DGF samples. (A) In situ hybridization analysis of miR-147 and immunostaining of KIM-1 in serial sections of sham control and cold storage-associated transplantation kidneys. Scale bar=50 μm. (B) Quantification of tubules according to miR-147 and KIM-1 staining. (C) qPCR analysis of miR-147 in Dicer WT and KO kidneys at 1, 7, or 14 days after cold storage-associated transplantation or sham surgery. (D) Cellular morphology and in RPTCs with or without CCCP-R treatment for indicated time. (E) Percentage of cell death assessed morphologically and qPCR analysis of miR-147. (F) In situ hybridization showing miR-147 upregulation in human grafts with DGF in comparison with normal (para-cancer) kidney tissues. Scale bar=50 μm. Quantitative data are expressed as mean±SD (n=3–5). Scale bar=100 μm. *P < 0.05 versus control. #P < 0.05 versus WT graft. Carbonyl Cyanide Chlorophenylhydrazone-Recovery, CCCP-R; DGF, delayed graft function; KO, knockout; miR-147, microRNA-147; RPTC, renal proximal tubular cell; WT, wild-type. Figure 3 can be viewed in color online at www.jasn.org.