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. 2023 May 22;34(8):1381–1397. doi: 10.1681/ASN.0000000000000154

Figure 7.

Figure 7

NDUFA4 preserves mitochondrial function and cell viability during ATP depletion injury in renal tubular cells. RPTCs were stably transfected with lentivirus containing NDUFA4 siRNA or scrambled sequence (A–D), or NDUFA4-pcDNA3.1 or empty pcDNA3.1 (E–H). The cells were then subjected to CCCP-induced ATP depletion treatment or control incubation. (A) Immunoblots showing NDUFA4 downregulation by its siRNA. (B) Immunoblots of cleaved caspase-3 and β-actin. (C) Cell morphology. Bar=100 μm. (D) The percentage of apoptosis evaluated morphologically. (E) Immunoblots verifying NDUFA4 overexpression in the cells transfected with NDUFA4-pcDNA3.1. (F) Immunoblots of cleaved caspase-3 and β-actin. (G) Cell morphology. Scale bar=100 μm. (H) The percentage of apoptosis evaluated morphologically. Quantitative data are expressed as mean±SD (n=4), *P < 0.05 versus control, #P < 0.05 versus CCCP-R treated groups transfected with scramble siRNA or empty pcDNA3.1. RPTCs, renal proximal tubular cells; siRNA, small interfering RNA. Figure 7 can be viewed in color online at www.jasn.org.