. 2023 Jul 19;101:skad244. doi: 10.1093/jas/skad244

Table 5.

Plasma concentrations of fatty acid methyl esters (µg/mL) on days 0, 35, 70, and 140 of cows offered no prepartum supplementation (NOSUP) and 1 kg/d of low-fat (LFAT) or high-fat (HFAT) bakery waste-based supplement from days 0 to 70 (6 pastures per maternal treatment; 6 cows and 4.3 ha per pasture)¹

Item²^,³	Day of the study				SEM	P-value
Item²^,³	0	35	70	140	SEM	Maternal treatment × day of the study
Stearic acid (18:0)
NOSUP	89^a	94^a	93^a	86^a	1.8	<0.01
LFAT	84^a	94^a	95^a	89^a	1.8
HFAT	86^a	93^a	92^a	96^b	1.8
Linoleic acid (18:2, ω-6)
NOSUP	184^a	185^a	200^a	183^a	7.6	<0.01
LFAT	183^a	197^a	222^b	190^a	7.6
HFAT	189^a	198^a	218^b	247^b	7.6
Total SFA
NOSUP	196^a	203^a	203^a	191^a	3.2	0.01
LFAT	188^a	202^a	207^a	197^a	3.2
HFAT	192^a	203^a	208^a	210^b	3.2
Total PUFA
NOSUP	206^a	209^a	230^a	211^a	7.6	<0.01
LFAT	204^a	220^a	249^b	219^a	7.6
HFAT	214^a	222^a	246^b	277^b	7.6
Total ω-6
NOSUP	191^a	193^a	209^a	190^a	7.4	<0.01
LFAT	195^a	204^a	230^b	198^a	7.4
HFAT	197^a	206^a	226^b	256^b	7.4
Total identified
NOSUP	431^a	449^a	469^a	435^a	9.4	<0.01
LFAT	420^a	459^a	492^b	451^a	9.4
HFAT	435^a	461^a	492^b	525^b	9.4

¹Cows calved their first offspring on average on day 89 ± 5. All pastures were offered free choice access to limpograss hay and supplemented with sugarcane molasses + urea (12 kg DM per cow per week) from days 110 to 236.

³SFA, saturated fatty acids; PUFA, polyunsaturated fatty acids; total ω-6 = linoleic (18:2), γ-linolenic (18:3), dihomo-γ-linolenic (20:3), arachidonic (20:4), docosadienoic (22:2), and osbond (22:5) acids.

²Plasma concentrations on day 0 of all fatty acids did not differ (P ≥ 0.23) among treatments but were covariate-adjusted for the respective plasma concentrations on day 0 (P ≤ 0.05).

^a,bWithin day of the study, means without common superscript differ (P ≤ 0.05).