Skip to main content
. 2023 Aug 3;7(8):e515. doi: 10.1002/pld3.515

FIGURE 2.

FIGURE 2

The MSL10 N‐terminus is disordered and structurally responds to a variety of environments in vitro. (a) Representative coomassie‐stained 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gel of his‐tagged MSL10N. His‐tagged MSL10N has an approximate molecular weight of 19 kDa. Circular dichroism spectra of his‐tagged MSL10N when exposed to increasing temperatures (b), TFE (c), PEG 400 (e), and glycerol (f). Spectra were obtained at 20°C in 20 mM sodium phosphate buffer, pH 7.4 unless otherwise specified. (d) The ratio of the mean residue ellipticity value at 222 and 208 nm for the indicated TFE treatments.