FIGURE 5.

MSL8 ^N forms condensates in response to salt and to low temperatures. (a) Fluorescence images of his‐tagged MSL8^N and MSL9^N with increasing levels of salt in 20 mM sodium phosphate. Protein concentration is in the upper left side of each image. (b) Image of tubes containing purified MSL8^N at the indicated temperatures. (c) Fluorescence images of MSL9^N and MSL8^N at room temperature (upper) and 4°C (lower). The samples in these images are in 50 mM NaCl, 20 mM sodium phosphate. The protein concentrations of MSL9^N and MSL8^N preparations were approximately 10 and 2.5 μM, respectively.