FIG. 2.

In vitro and in vivo expression of UL28. Equivalent amounts of in vitro-translated UL28 were immunoprecipitated with either preimmune (A) or anti-UL28 (B) antisera or left neat (C). Vero cells were infected with HSV(KOS) at an MOI of 5, radiolabeled with [³⁵S]methionine (New England Nuclear) between 6 and 9 h postinfection, and then harvested at 16 h as described previously (13). Cell extracts were immunoprecipitated with either preimmune (D) or anti-UL28 H85 (E) antisera. The mobilities of molecular mass markers are indicated to the left. UL28 and the 81-kDa protein are indicated to the right.