FIG. 1.

Recognition of an HLA-A2-restricted peptide in A clade p17 by patient 1 CTLs. (a) Gag-specific CTLs from donor 1 specifically lysed HLA-A2-matched target cells pulsed with multiple variants of the A clade version of the previously defined HLA-A2-restricted peptide in p17, but not the B clade index version (all peptides were prepulsed for 1 h at 10 μM). (b) p17-specific CTLs from patient 1 were stained with the HLA-A*0201 tetramer complexed with either the B clade version (Y3; shown as 3Y) of the known p17 epitope peptide or the A clade version (F3; shown as 3F). The plots show the mononuclear cell population costained with tetramer (phycoerythrin [PE]) and anti-CD8 (fluorescein isothiocyanate). A double-positive population is seen only with the F3 tetramer.