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. 1999 Feb;73(2):1708–1714. doi: 10.1128/jvi.73.2.1708-1714.1999

FIG. 2.

FIG. 2

FIG. 2

FIG. 2

FIG. 2

FIG. 2

Recognition of A clade Gag peptides through HLA-A24 and -Cw4 by patient 2 CTLs. (a) Bulk CTL cultures from donor 2 were tested for recognition of autologous and target cell lines matched through a single class I HLA molecule presenting two 20-mer A clade Gag peptides (used at 10 μM; E/T ratio, 50:1). (b) Patient 2 bulk CTL cultures were tested for recognition of target cells matched through HLA-A24 pulsed with peptides representing truncations of the B clade version of the epitope containing residues 295 to 314 (used at 10 μM; E/T ratio, 36:1). (c) A CTL line from donor 2 specific for the HLA-A24-restricted peptide was tested for recognition of HLA-A24-matched targets with various concentrations of the A and B clade peptides in the assay (E/T ratio, 25:1). (d) A CTL line from donor 2 specific for the HLA-Cw-4-restricted p17 peptide was tested for recognition of autologous targets presenting either the A clade 20-mer or the equivalent B clade 20-mer (peptides used at 10 μM). (e) The same p17-specific CTL line from donor 2 was used to study recognition of target cells presenting either the A or B clade 20-mers at different concentrations in the assay.