Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 10;26(8):1365–1378. doi: 10.1038/s41593-023-01377-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, Cryogenic electron microscopy structure of RyR2 (gray, top and side view) showing the location of the Ser2808 in the RY3&4 phosphorylation domain (magenta) and calstabin2 (cyan). RyR2 PKA phosphorylation shifted the channel toward a primed state (yellow)⁵⁰. b,c, Representative SDS–PAGE analysis and quantification of modified RyR2 and calstabin2 immunoprecipitated from hippocampal RyR2 complex (IP RyR2; bands normalized to total RyR2) in SHAM (n = 6), MI (n = 6), MI + ARM036 (n = 6), MI + S107 (n = 6), MI+ propranolol (n = 4) and MI + SD-208 (n = 4) mice. d, Single-channel traces of RyR2 incorporated in planar lipid bilayers with 150 nM Ca²⁺ in the cis chamber, corresponding to representative experiments performed with hippocampal samples from SHAM (n = 6), MI (n = 5), MI + ARM036 (n = 6), MI + S107 (n = 5), MI + propranolol (n = 5) and MI + SD-208 (n = 5) mice. e, RyR2 P_o, T_o and T_c in the same groups. f, Ca²⁺ leak measured in microsomes from mouse hippocampi of the same groups. g, Bar graphs represent the quantification of Ca²⁺ leak as the percentage of uptake in SHAM (n = 6), MI (n = 6), MI + ARM036 (n = 6), MI + S107 (n = 6), MI + propranolol (n = 3) and MI + SD-208 (n = 3) mice. Individual values are shown with the mean ± s.e.m. One-way ANOVA and Tukey’s test post hoc correction for multiple comparisons shows *P < 0.05, SHAM versus MI or MI + ARM036; ^#P < 0.05, MI versus MI + S107, MI + propranolol or MI + SD-208. Data are derived from biologically independent samples. All statistical tests were two sided.

Source data