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. 2023 Jul 20;14:1197650. doi: 10.3389/fimmu.2023.1197650

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Copyright © 2023 Assil, Buters, Hameeteman, Hallard, Treijtel, Niemeyer – Van der Kolk, de Kam, Florencia, Prens, van Doorn, Rissmann, Klarenbeek, Jansen and Moerland

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prednisolone suppresses the NF-κB driven cytokines and IRF7 driven response in blister fluid. (A) IL-6 (B) IL-8 (C) TNF (D) IL-1b (E) IL-10 (F) Mx-A. Cytokine concentrations in blister fluid were analysed by MSD and Mx-A by ELISA. Data are presented as mean ± SD. For IL-6, IL-1b and Mx-A no statistical model was applied as the majority of the values were <LLOQ in the prednisolone group. ELISA, enzyme- linked immunosorbent assay; IL, interleukin; LLOQ, lower limit of quantification; MSD, meso scale discovery; TNF, tumor necrosis factor; TS, tape stripped.