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. 2023 Jul 20;14:1197650. doi: 10.3389/fimmu.2023.1197650

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Copyright © 2023 Assil, Buters, Hameeteman, Hallard, Treijtel, Niemeyer – Van der Kolk, de Kam, Florencia, Prens, van Doorn, Rissmann, Klarenbeek, Jansen and Moerland

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ex-vivo whole blood stimulated with 20 μg/ml IMQ. (A) IL-1b (B) IL-6 (C) IFN-g. Cytokine concentrations in blood were analysed by V-plex and MSD. Pre-dose time point refers to sample taken prior to prednisolone/placebo dosing. The time point 48h refers to sample taken at 48h after initial dose but before the 5th dose of prednisolone/placebo. Sample taken at 52h refers to time point 4 hours after 5th dose. At 96h after prednisolone/ placebo, the sample was taken before the 8th dose. IFN, interferon; IL, interleukin; MSD, meso scale discovery.