TABLE 1.
Induction of cytotoxic T-cell responses to BDV-specific proteins by VV-BDV recombinants
| Exptd | Recombinant virus used for infectiona | % Specific lysisb of F10 target cells infected with:
|
||
|---|---|---|---|---|
| VV | BDVe | NLc | ||
| I | VV-p24 | 65, 60, 33, 14 | 11, 16, 10, 5 | 5, 1, 1, 2 |
| VV-p40 | 84, 74, 30, 13 | 41, 21, 13, 9 | 0, 1, 2, 1 | |
| VV-gp18 | 50, 44, 24, 7 | 14, 13, 10, 1 | 2, 1, 0, 0 | |
| VV-gp94 | 46, 48, 28, 8 | 29, 13, 11, 3 | 0, 1, 0, 0 | |
| II | VV-p24 | 49, 32, 19, 8 | 3, 7, 0, 0 | 3, 8, 4, 2 |
| VV-p40 | 57, 35, 20, 9 | 26, 19, 9, 3 | 0, 2, 1, 0 | |
| VV-gp18 | 53, 34, 16, 10 | 5, 12, 7, 11 | 1, 9, 5, 1 | |
| VV-gp94 | 51, 63, 41, 16 | 32, 26, 16, 6 | 0, 1, 0, 0 | |
| III | VV-p24 | 45, 24, 9, 5 | 18, 9, 3, 0 | 12, 8, 3, 0 |
| VV-p40 | 62, 29, 14, 3 | 35, 21, 9, 3 | 7, 9, 2, 2 | |
| VV-gp18 | 47, 20, 5, 2 | 0, 3, 2, 0 | 1, 2, 1, 2 | |
| VV-gp94 | 40, 25, 7, 0 | 24, 14, 4, 4 | 11, 7, 3, 1 | |
a
Six- to eight-week-old female Lewis rats were infected i.v. with 107 PFU of VV-BDV recombinants and killed 6 days later. Values of specific lysis are mean values.
b
Results shown for effector/target cell ratios of 30:1, 10:1, 3:1, and 1:1, respectively.
c
NL, noninfected F10 cells.
d
Experiments were performed independently. Two animals were infected with the respective VV-BDV recombinant in all experiments.
e
Boldface values indicate VV-BDV recombinant-specific lysis of BDV-infected F10 target cells.