TABLE 2.
Lytic activity of effector cells on VV-BDV recombinant virus-infected F10 cells
| Effector cellsa | % Specific lysis of F10 target cells infected with:
|
||||||
|---|---|---|---|---|---|---|---|
| BDV | VV | NLb | VV-p24 | VV-p40e | VV-gp18 | VV-gp84/94 | |
| Expt Ic | |||||||
| BDV | 80, 66, 25 | 0, 1, 0 | 0, 2, 0 | 6, 0, 0 | 76, 42, 9 | 4, 1, 0 | 5, 0, 0 |
| VV | 3, 2, 0 | 58, 38, 24 | 4, 1, 0 | 62, 33, 12 | 68, 31, 28 | 66, 41, 17 | 57, 37, 21 |
| Expt IIc | |||||||
| BDV | 69, 22, 0 | 0, 0, 0 | 3, 1, 0 | 3, 1, 0 | 100, 68, 32 | 0, 1, 0 | 0, 0, 0 |
| VV | 7, 6, 5 | 79, 43, 18 | 5, 9, 0 | 59, 36, 17 | 63, 37, 28 | 56, 31, 17 | 54, 30, 14 |
| Expt IIId | |||||||
| BDV | 100, 70, 32, 17 | 0, 2, 1, 0 | 2, 2, 1, 0 | 3, 2, 3, 0 | 81, 60, 25, 8 | 2, 0, 0, 0 | 13, 4, 0, 0 |
| VV | 2, 7, 5, 2 | 52, 34, 13, 3 | 5, 5, 4, 0 | 46, 38, 12, 4 | 53, 44, 16, 7 | 56, 50, 23, 9 | 49, 42, 17, 0 |
a
Effector cells were isolated from the brain of BDV-infected Lewis rats 19 days p.i. and cultivated overnight (BDV), or female Lewis rats were infected i.v. with 107 PFU of VV and spleen cells were taken 6 days p.i. (VV).
b
NL, noninfected F10 cells.
c
Results are shown for effector/target cell ratios of 10:1, 3:1, and 1:1, respectively, for BDV and 100:1, 30:1, and 10:1, respectively, for VV.
d
Results are shown for effector/target cell ratios of 20:1, 7:1, 2:1, and 0.7:1, respectively, for BDV and 100:1, 30:1, 10:1, and 3:1, respectively, for VV.
e
Boldface values indicate BDV-specific lysis of F10 target cells infected with VV-BDV recombinants.