Fig. 3.

Direct comparison of protein expression changes in young vs. old muscle following resistance exercise training. Panel A: heatmap depicting the similarities/differences in protein expression changes induced by resistance exercise training (RET) in old (O) vs. young (Y) muscle. Red and blue shading denote upregulation and downregulation, respectively. Panel B: representative enriched Gene Ontology and biological pathway (KEGG/Reactome) terms for the various different permutations depicted in panel A. Strength of colour shading depicts magnitude of enrichment significance, given by the negative log10 of that term’s enrichment false discovery rate (FDR) P-value (with darker shading analogous with a stronger FDR P-value). Numbers of enriched proteins in each case are given in associated heatmap boxes. Panel C: skeletal muscle-specific minimum interaction networks for proteins regulated by RET in young muscle that are not regulated in the same manner by RET in old muscle. In each case, yellow nodes represent differentially regulated proteins whilst grey nodes represent other strongly interacting components of the proteome. Red borders depict hub proteins in each instance, with the top ranked hub given by a larger node size