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. 2023 Aug 3;14(8):495. doi: 10.1038/s41419-023-06027-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Immunoblots of B7-H3 treated with or without PNGase F at 37 °C for 3 h. B Immunoblots of truncated B7-H3 containing the first IgV-IgC domain (aa1-258) treated with or without PNGase F at 37 °C for 3 h. C Immunoblots of truncated B7-H3 containing N91Q, N104Q, N189Q or N215Q mutation. D AOL blot of Flag-B7-H3 immunoprecipitated by anti-Flag beads. E IHC stains of AOL and B7-H3 in CRC tissues. F Immunoblots of B7-H3 in SW480 and HCT-8 cells upon knockdown of each fucosyltransferase. G Immunoblots of membranous AOL and B7-H3 in SW480 and HCT-8 cells upon FUT8 knockdown. H Immunoblots of B7-H3 and its downstream AKT/MTOR pathway molecules in SW480 and HCT-8 cells upon FUT8 knockdown. All experiments were performed in technical triplicates and are displayed as mean ± s.d.; ns no significance; *P < 0.05, **P < 0.01, ***P < 0.001.