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. 2023 Aug 3;14(8):495. doi: 10.1038/s41419-023-06027-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Immunoblots of B7-H3 protein in SW480 and HCT-8 cells after knockdown of FUT8 and / or HSC70. B Immunoblots of B7-H3 protein in SW480 and HCT-8 cells after knockdown of FUT8 and / or LAMP2A. C Co-IP assays showed the effects of FUT8 knockdown on the interaction between B7-H3 and HSC70 or LAMP2A. D Schematics of truncated B7-H3 (aa 1-258) with the N104 glycosylation site and 106-110SLRLQ motif for HSC70 binding, and AOL blots of the co-IP B7-H3 protein with wildtype N104 and N91Q/N189Q/N215Q mutations. E Schematics of truncated B7-H3 (aa 1-258) with N104Q or Q110A mutation, and immunoblots of truncated B7-H3 in SW480 cells that were treated with 100 μM CHX after transfection of B7-H3 expression plasmids containing N104Q or Q110A mutation. F Schematics of truncated B7-H3 (aa 1-258) with N104Q or VRV112-114NAS mutation, and immunoblots of truncated B7-H3 containing N104Q and / or VRV112-114NAS mutation in SW480 cells. G Co-IP assays showed the interaction between the truncated B7-H3 containing N104Q or Q110A mutation and HSC70 or LAMP2A. All experiments were performed in technical triplicates and are displayed as mean ± s.d.; ns no significance; *P < 0.05, **P < 0.01, ***P < 0.001.