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. 2023 Aug 3;14:4417. doi: 10.1038/s41467-023-40094-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative gating strategies used in cholesteatoma and skin samples. Live (calcein⁺ AAD⁻) CD45⁻ cells. Scale bars: 5 mm. b UMAP plot of scRNA-seq data from 19,273 cells labeled by sample condition. Samples were obtained from three pairs of cholesteatoma and control skin samples labeled according to sample condition. c UMAP plot of scRNA-seq data labeled according to cell type identified in PanglaoDB. The main cell types were keratinocytes, fibroblasts, and endothelial cells.