Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 3;14:4668. doi: 10.1038/s41467-023-40047-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Differential susceptibilities to CHIKV-heart infection between MyD88^−/−, Trif ^Lps2/Lps2, Casp1/11^−/−, Mavs^−/−, and WT mice. WT: 2 dpi (n = 10), 5 dpi (n = 16), and 7 dpi (n = 6). Casp1/11^−/−: 2 dpi (n = 5), 5 dpi (n = 8), and 7 dpi (n = 3). MyD88^−/−: 2 dpi (n = 4), 5 dpi (n = 6), and 7 dpi (n = 3). Trif ^Lps2/Lps2: 2 dpi (n = 5), 5 dpi (n = 5), and 7 dpi (n = 4). Mavs^−/−: 2 dpi (n = 4), 5 dpi (n = 4), and 7 dpi (n = 4). b, c CHIKV-heart infection kinetics in Mavs^−/− and WT mice. b WT: 2 dpi (n = 6), 5 dpi (n = 7), 7 dpi (n = 4), 10 dpi (n = 4), and 15 dpi (n = 2). Mavs^−/−: 2 dpi (n = 5), 5 dpi (n = 6), 7 dpi (n = 10), 10 dpi (n = 8), and 15 dpi (n = 4). c. WT: 2 dpi (n = 6), 5 dpi (n = 7), 7 dpi (n = 4), 10 dpi (n = 4), and 15 dpi (n = 2). Mavs^−/−: 2 dpi (n = 5), 5 dpi (n = 6), 7 dpi (n = 8), 10 dpi (n = 5), and 15 dpi (n = 4). CHIKV RNA genomes from heart (d) and from calf muscle homogenates (e). d HI-control (n = 6). WT: 1 dpi (n = 5), 2 dpi (n = 6), 10 dpi (n = 4), and 15 dpi (n = 2). Mavs^−/−: 1 dpi (n = 9), 2 dpi (n = 5), 10 dpi (n = 8), and 15 dpi (n = 4). e HI-control (n = 3). WT: 2 dpi (n = 4), 10 dpi (n = 5), and 15 dpi (n = 3). Mavs^−/−: 2 dpi (n = 4), 10 dpi (n = 8), and 15 dpi (n = 4). f Left panel: CHIKV RNA levels in top and bottom heart sections at 10 dpi. HI-control (n = 1), WT (n = 3), and Mavs^−/− (n = 3). Right panel: ratio of viral RNA levels between top and bottom heart sections. N = 3/group. WT dataset at 10 dpi is shared with Fig. 1c. g Isg15 expression levels in Mavs^−/− and WT CHIKV-infected hearts. WT: HI-control (n = 4), 12 hpi (n = 4), 24 hpi (n = 5), and 2 dpi (n = 6). Mavs^−/−: HI-control (n = 4), 12 hpi (n = 9), 24 hpi (n = 9), and 2 dpi (n = 7). Protein levels of IFN-α and INF-β in heart (h) and serum (i) at 24 hpi. h. HI-control (n = 7), WT (n = 5), and Mavs^−/− (n = 9). i. HI-control (n = 7), WT (n = 5), and Mavs^−/− (n = 7). Data shown as mean ± SEM (a, d-g). Boxplots show median and quartile ranges, whiskers represent the range (b, c, h, i). P values were calculated using two-tailed Mann-Whitney test (a–i). Created with BioRender.com. Source data are provided as a source data file.