Figure 5.

Transcriptional activity of novel POU4F3 variants. (a) Luciferase activities measured under specific conditions. To minimize the ceiling effect, the condition (i.e., empty‐Luc 2 µg and SNAP25‐Luc 2 µg) was determined as the luciferase vector system. (b,c) The transcriptional activities of the wild-type and mutant POU4F3 proteins in the SNAP25-Luc vector were normalized to that of the internal control (Myc-DDK). Using the luciferase vector system, the transcriptional activity in the wild-type and the four POU4F3 variants were analyzed. All variants exhibited significantly reduced transcriptional activities compared to the wild type. Experiments were conducted in duplicate, and measurements were taken three times to determine luciferase activity. Consequently, the sample size for each experiment was six. The four mutant constructs corresponding to each variant differentiated by employing distinct colors for clear visualization. *, a statistical significance by one-way ANOVA and Bonferroni’s multiple comparison test.