Figure 6.

Immunofluorescence of the wild-type and mutant POU4F3 proteins. (a) Cells were immuno-stained with anti-Myc (green) and phalloidin (red). The nuclei were stained with DAPI (blue). (b) Cells were immuno-stained with anti-DDK (green) and Rhodamine-phalloidin (red). Rhodamine-phalloidin (red) staining was used to label F-actin and stabilize actin filaments in vitro. The nuclei were stained with DAPI (blue). Upon examination through confocal microscopy, the region where the green fluorescence (representing the target protein) and the blue fluorescence (DAPI-stained nuclei) overlap appears as a turquoise color. (c) Quantitation of cytoplasmic and nuclear localization of POU4F3, depending on the variants.