Fig. 1.

Neonatally engrafted control and HD patient-derived human neural progenitor cells differentiate into striatal neurons. a–l Striatal coronal sections from CTR-33 and HD-60 chimeric brains at 1 month PST immuno-labelled for GFP, hNA or STEM121 (green) and MAP2 (a, b), NeuN (c, d), Olig2 (e, f), CTIP2 (g, h), DARPP-32 (i, j) or Calretinin (Calret) (k, l) (red). m–r Striatal coronal sections from CTR-33 and HD-60 chimeric brains at 3 months PST immunolabelled for STEM121 or GFP (green) and DARPP-32 (m–r) or Calret (q, r) (red). s–w Histograms representing the percentage of hNA⁺ cells expressing NeuN (s), Olig2 (t), CTIP2 (u), DARPP-32 (v) and Calret (w) in CTR-33 and HD-60 chimeric brains at 1, 3 and 5 months PST. M, months. Scale bars 20 µm in o; 50 µm in a–e, g, i, k, m, q; 100 µm in j. Data are expressed as mean ± SEM. s, t n = 5 mice; Unpaired t-test, two-tailed, non-significant. u n = 5 mice; Unpaired t-test, two-tailed, P = 0.0465 (CTR 5 M vs HD 5 M), P = 0.0051 (HD 1 M vs HD 3 M), P = 0.0133 (HD 3 M vs HD 5 M), P = 0.0002 (HD 1 M vs HD 5 M). v n = 5 mice; Unpaired t-test, two-tailed, P = 0.0273 (CTR 1 M vs HD 1 M), P = 0.0489 (HD 1 M vs HD 3 M), P = 0.0254 (HD 3 M vs HD 5 M), P = 0.0022 (HD 1 M vs HD 5 M). w n  = 5 mice; Unpaired t-test, two-tailed, P = 0.0451 (CTR 5 M vs HD 5 M), P = 0.0117 (HD 1 M vs HD 3 M), P = 0.0072 (HD 3 M vs HD 5 M), P < 0.0001 (HD 1 M vs HD 5 M). See also Supplementary Table 2