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. 2023 Jul 28;120(31):e2215632120. doi: 10.1073/pnas.2215632120

Fig. 3.

Fig. 3.

Characterization of TADA ASD risk genes at FDR < 0.1 during development. (A) Mean expression and percentage of cells expressing TADA genes from single-cell data of the human fetal brain cortex. Asterisks indicate cell type–specific TADA genes (Materials and Methods). (B) Cell types enriched in TADA genes tested using Expression Weighted Cell Type Enrichment (EWCE). Asterisks indicate FDR < 0.05. (C) TADA genes’ variation during development using long-term maturation human cortical spheroids (hCSs) and BrainSpan. Genes are stratified by those with a majority of inherited or de novo support. (D) Clustering of the TADA genes from hCS trajectory data identified three clusters. Days postconception, TADA class (majority of inherited or de novo), and scaled expression are shown. The average expression of each cluster and significant gene ontology biological terms are shown. vRG = ventral radial glia, PgS = cycling progenitor (S phase), PgG2M = cycling progenitor (G2M phase), Per = pericyte, oRG = outer radial glia, OPC = oligodendrocyte precursor, Mic = microglia, IP = intermediate progenitor, InMGE = interneuron medial ganglionic eminence, InCGE = interneuron caudal ganglionic eminence, ExN = excitatory neuron, ExM−U = maturing excitatory neuron upper enriched, ExM = maturing excitatory neuron, ExDp2 = excitatory neuron deep layer 2, ExDp1 = excitatory neuron deep layer 1, End = endothelial, ExMig = migrating excitatory neuron, ExDp = excitatory neuron deep.