REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-TH; 1:1000	Sigma-Aldrich	Cat#MAB318; RRID: AB_2201528)
Rabbit polyclonal anti-TH; 1:1000	PhosphoSolutions	Cat#2025-THRAB; RRID: AB_2492276)
Rabbit polyclonal anti-MAP2 polyclonal; 1:1000	Cell Signaling Technology	Cat#4542S; RRID: AB_10693782
Mouse monoclonal anti-MAP2 (2a+2b); 1:1000	Sigma-Aldrich	Cat#M1406; RRID: AB_477171
Goat polyclonal anti-mouse IgG AF488; 1:400	Thermo Fisher	Cat#A-11029; RRID: AB_2534088)
Goat polyclonal anti-rabbit IgG AF594, 1:400	Thermo Fisher	Cat#A11037; RRID: AB_2534088)
Plasmids
TetO-NGN2-T2A-puromycin plasmid	Dr. Thomas C. Südhof laboratory3,8	N/A
Ubi-rtTA plasmid	Dr. Thomas C. Südhof laboratory3,8	N/A
Chemicals, peptides, and recombinant proteins
Accutase	STEMCELL Technologies	Cat#07920
Antibiotic-Antimycotic (100×)	Thermo Scientific	Cat#15240062
B-27 Supplement (50×) serum free	Thermo Scientific	Cat#17504044
BDNF Recombinant Human/Murine/Rat	PeproTech	Cat#450-02
Boric acid	Sigma-Aldrich	Cat#B6768
Bovine serum albumin (BSA)	BioShop	Cat#ALB001.500
Cytosine β-D-arabinofuranoside hydrochloride (Ara-C)	Sigma-Aldrich	Cat#C6645
DMEM/F-12 GlutaMAX	Thermo Fisher	Cat#10565018
Doxycycline	Takara Bio Company	Cat#631311
Dulbecco’s phosphate-buffered saline (DPBS)	Thermo Fisher	Cat#14190250
DPBS calcium magnesium (Ca+ Mg+)	Thermo Fisher	Cat#14040182
Ethylenediaminetetraacetic acid (EDTA)	Fisher Scientific	Cat#E478-500
Fetal bovine serum (FBS)	Sigma-Aldrich	Cat#F1051
Fluoromount-G Mounting Medium	Thermo Fisher	Cat#00-4958-02
GDNF Recombinant Human/Murine/Rat	PeproTech	Cat#450-10
GlutaMAX Supplement	Thermo Fisher	Cat#35050061
Human Recombinant Sonic Hedgehog (C24II) (Shh)	STEMCELL Technologies	Cat#78065
Laminin Mouse Protein	Thermo Fisher	Cat#23017015
Matrigel basement membrane matrix LDEV-free	Corning	Cat#354277
MEM Nonessential Amino Acid solution 100× (NEAA)	Wisent Bioproducts	Cat#321-011-EL
mFreSR	STEMCELL Technologies	Cat#05854
mTeSRTM Plus	STEMCELL Technologies	Cat#100-0276
N-2 Supplement (100×)	Thermo Scientific	Cat#17502048
Neurobasal Medium	Thermo Scientific	Cat#21103049
Nitric acid ACS reagent 70%	Sigma-Aldrich	Cat#438073
Normal goat serum	Wisent Bioproducts	Cat#053-110
Paraformaldehyde (PFA)	Electron Microscopy Sciences	Cat#19210
PBS 10× solution	Fisher Scientific	Cat#BP399-20
Poly-L-ornithine hydrobromide mol wt 30,000–70,000	Sigma-Aldrich	Cat#P3655
Potassium chloride (KCl)	Sigma-Aldrich	Cat#P3911
Puromycin 2HCL	Wisent Bioproducts	Cat#400-160-EM
STEMdiff Midbrain Neuron Differentiation Kit	STEMCELL Technologies	Cat#100-0038
STEMdiff Midbrain Neuron Maturation Kit	STEMCELL Technologies	Cat#100-0041
Sodium chloride (NaCl)	Sigma-Aldrich	Cat#S7653
Sucrose ≥99.5% (GC)	Sigma-Aldrich	Cat#S9378
Triton X-100	Sigma-Aldrich	Cat#T8787
Trypan blue	Bio-Rad	Cat#1450022
Y-27632 (ROCK inhibitor)	Tocris	Cat#1254
DAPI	Thermo Fisher Scientific	Cat#D3571
Experimental models: Cell lines
Human Induced Pluripotent Stem Cells (hiPSC), cell line: AIW002-02. Approved by the CHU de Quebec Research Center (#2022-6079)	Obtained through the C-BIG Repository at the Montreal Neurological Institute (The Neuro)	N/A
Critical commercial assays
qPCR Lentivirus Titer Kit	Applied Biological Materials Inc.	Cat#LV900
Software and algorithms
ImageJ	N/A	https://imagej.nih.gov/ij/
GraphPad Prism 9.0	GraphPad Software Inc.	https://www.graphpad.com/scientific-software/prism/
Other
German glass coverslips #1.5 (8 mm)	Electron Microscopy Sciences	Cat#72296-08