REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100)	BioLegend	Cat#103116
BV605 anti-mouse CD31 (1:100)	BioLegend	Cat#102427
Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100)	BD Pharmingen™	Cat #553142
Chemicals, peptides, and recombinant proteins
10× Tris buffered saline (TBS)	Bio-Rad	Cat#1706463
16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade	Thomas Scientific	Cat# C993M26
Ammonium chloride (NH4Cl), crystal	VWR Chemicals	Cat#12125-02-9
Fetal bovine serum (FBS)	Gibco	Cat#10437-028
DAPI (1:100)	BioLegend	Cat#422801
PBS (10×), pH 7.4	Thermo Fisher Scientific	Cat#70011069
PBS (1×), pH 7.4	Thermo Fisher Scientific	Cat#10010023
Percoll	Sigma-Aldrich	Cat#17-0891-02
Prolong™ Gold antifade reagent with DAPI	Invitrogen	Cat#P36980
RPMI 1640 Medium	Thermo Fisher Scientific	Cat#11875093
Sodium azide, 5%	Fisher Scientific	Cat#71448-16
Triton X-100	American Bio	Cat#9002-93-1
UltraPure 0.5 M EDTA, pH 8.0	Life Technologies	Cat#15575020
Other
Cover Glass Thickness 1 ½ 22 × 22 mm	Corning	Cat#2850-22
Epredia™ Single Cytofunnel™ with White Filter Cards	Thermo Fisher Scientific	Cat#5991040
Epredia™ Cytoclip™ Stainless-Steel Slide Clip	Thermo Fisher Scientific	Cat#59910052
Glass Coplin Jar	Thermo Fisher Scientific	Cat#E94
Unifrost Plus Microscope Slides Adhesive, Treated Surfaces	Azer Scientific	Cat#EMS200G+
ImmEdge® Hydrophobic Barrier PAP Pen	Vector Laboratories	Cat#H-4000
Thermo Scientific™ Cytospin™ 4 Cytocentrifuge	Thermo Fisher Scientific	Cat# A78300003
Sorvall Legend T Benchtop Centrifuge Rotor: 75006445	Sorvall Legend	Cat#SO-LEGT
BD FACS Aria II sorter	BD Biosciences	N/A
Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor	Beckman Coulter	Cat#BE-A6
Leica TCS SP8	Leica	N/A
Software and algorithms
FlowJo 10	BD	http://flowjo.com