Fixative Solution: 4% Paraformaldehyde
| Reagent | Final concentration | Volume |
|---|---|---|
| 16% PFA | 4% | 2.5 mL |
| 10× PBS | 1× | 1 mL |
| ddH2O | N/A | 6.5 |
| Total | N/A | 10 mL |
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