REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental models: Organisms/strains
Hydra vulgaris (interstitial GCaMP6s transgenic)	Hydra vulgaris AEP strain	Dupre and Yuste5
Hydra vulgaris (Hym-355 GFP transgenic)	Hydra vulgaris AEP strain	Yamamoto and Yuste6
Hydra vulgaris (Hym-355 GCaMP6s transgenic)	Hydra vulgaris AEP strain	Yamamoto and Yuste6
Recombinant DNA
pHyVec1	Addgene	Addgene_34789
GCaMP6s Synthetic	GeneArt	Custom
Hym-355 promotor/terminator	GENEWIZ	Custom
Software and algorithms
Codes used for the paper	GitHub	https://github.com/NTCColumbia
Instructions to install DeepLabCut	GitHub	https://deeplabcut.github.io/DeepLabCut/docs/installation.html
Other
Fluorescent stereo microscope	Leica	M165
ORCA-Flash 4.0 LT3 Digital CMOS Camera	Hamamatsu	C11440-42U40
Leica EL6000 External light source	Leica	EL6000
VWR micro cover glass 22 × 22 mm	VMR	48366-067
SecureSeal™ Imaging Spacers	GRACE BIO-LABS	SKU: 654002
Two-photon laser-scanning microscope, Ultima In Vivo	Prairie Technologies/Bruker	N/A
Ti:sapphire laser	Coherent	Chameleon Ultra II
50–160-BK Pockels cell	Conoptics	302RM
Electrically tunable lens	Optotune AG	EL-10-30-C-NIR-LD-MV
Pulse-amplified laser	Spectra-physics	Spirit 1040–8
1147-4-1064 Pockels cell	FastPulse Technology	8025RS-H-2KV
Data acquicition system, Multifunction I/O Device	National Instruments	USB-6008
USB digital microscope	Bysameyee	Microscope-1000×
25× objective	Olympus	XLPLN25XWMP2
Autofluorescent plastic slides	CHROMA	92001
FB650-40 - Ø1″ Bandpass Filter	THORLABS	FB650-40
USB digital microscope	Bysameyee	Microscope-1000×