Activated Papain (40 μL per 1 mg of IgG)

Reagent	Final concentration	Amount
Papain	1% (w/w)	38.5 μL (at 26 mg/mL)
10× Tris/EDTA	1×	100 μL
10× L-cysteine	1×	100 μL
ddH2O	N/A	761.5 μL
Total	N/A	1 mL