Fig. 2.

GPCR specificity of DFPQ. HEK 293 cells were cotransfected with β-arrestin2-GFP10 and either β₂AR-RlucII (A), β₁AR-RlucII (B), or CXCR4-RlucII (C) for 48 h and cells were preincubated with 0.1% DMSO (negative control) or 10 μM DFPQ for 30 min. Cells were then incubated with Coelenterazine 400a for 2 min and stimulated with 1 μM of the indicated agonist. Cells were read every 2 min post agonist addition. One-way ANOVA with multiple comparisons was performed to compare the means of each treatment group. Data are the mean ± SEM, n = 3. ****P < 0.0001; ns, not significant