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. 2023 Jun 16;10(22):2301110. doi: 10.1002/advs.202301110

Figure 1.

Figure 1

Inhibition of MS‐DG pathway ameliorates depressive‐like behavior of mice. A) Experimental timeline of CSDS protocol and behavioral tests. B) Bilateral delivery of retrograde AAV‐expressing Cre‐recombinase in the DG and AAV carrying Cre‐dependent hM4Di‐mCherry into the MS of C57BL/6J mice. C) mCherry labeling (red) showing hM4Di‐expressing neuron in the DG at low (left) and high (right) magnification. Scale bar: 200 µm (left), 20 µm (right). D) Effects of chemogenetic inhibition of MS‐DG neurons on social interaction ratio in SIT. n = 12–15 per group. E) Effects of chemogenetic inhibition of MS‐DG neurons on the immobility time in TST. n = 12–15 per group. F) Effects of chemogenetic inhibition of MS‐DG neurons on the immobility time in FST. n = 12–15 per group. G) Effects of chemogenetic inhibition of MS‐DG neurons on the locomotor activity in OFT. n = 12–15 per group. H) Schematic of SSDS protocol detailing the timing for hM3Dq expression and behavioral tests. I) Bilateral delivery of retrograde AAV expressing Cre‐recombinase in the DG and AAV carrying Cre‐dependent hM3Dq‐mCherry into the MS of C57BL/6J mice. J) mCherry labeling (red) showing hM3Dq‐expressing neuron in the DG at low (left) and high (right) magnification. Scale bar: 200 µm (left), 20 µm (right). K) Effects of chemogenetic activation of MS‐DG neurons on social interaction ratio in SIT. n = 19–20 per group. L) Effects of chemogenetic activation of MS‐DG neurons on the immobility time in TST. n = 19–20 per group. M) Effects of chemogenetic activation of MS‐DG neurons on the immobility time in FST. n = 19–20 per group. N) Effects of chemogenetic inhibition of MS‐DG neurons on the locomotor activity in OFT. n = 19–20 per group. Data are expressed as mean ± SEM. Two‐way ANOVA followed by the Bonferroni's post hoc test (D–G), Student's t‐test (K–N). *p < 0.05, **p < 0.01, ***p < 0.001. The statistical details can be found in Table S4, Supporting Information.