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. 2023 May 18;10(22):2300758. doi: 10.1002/advs.202300758

Figure 3.

Figure 3

CSC NPs ameliorated MPP+ ‐induced mitochondrial dysfunction through the SIRT1/PGC‐1α pathway. a) CLSM images indicated the expression of SIRT1 and PGC‐1α proteins in SH‐SY5Y cells pretreated with different concentrations of CSC NPs and then incubated with MPP+. Blue: cell nuclei; green: SIRT1; red: PGC‐1α. b) Western blot and c,d) quantification of SIRT1 and PGC‐1α proteins in SH‐SY5Y cells pretreated with different concentrations of CSC NPs and incubated with MPP+ (n = 3). e–h) Gene expressions of PGC‐1α, PPARγ, NRF1, and mitochondrial copy number (mtDNA CN) analyzed by qRT‐PCR (n = 3). i) Schematic illustration of the mechanism of CSC NPs improving mitochondrial dysfunction and biogenesis via SIRT1/PGC‐1α pathway. The two‐sided one‐way ANOVA with a Tukey post hoc analysis was used for comparison among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.