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. 2023 Apr 17;29(9):2690–2704. doi: 10.1111/cns.14213

FIGURE 4.

FIGURE 4

Knockdown or upregulation of the transcription factor ZNF148 suppressed or promoted the malignant phenotype of t‐DCs, respectively. (A, B) The transfection efficiency was verified by qRT‐PCR and western blot. (C–E) CCK8 and clone formation assay of t‐DCs transfected with si‐NC, si‐ZNF148, Vector, or ZNF148‐overexpression plasmids. (F, G) Transwell and wound healing assay of t‐DCs transfected with si‐NC, si‐ZNF148, Vector, or ZNF148‐overexpression plasmids. (H) Tumorigenicity assay in nude mice after subcutaneous inoculation of shZNF148 or ZNF148 t‐DCs. Data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.