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. 2023 Apr 17;29(9):2690–2704. doi: 10.1111/cns.14213

FIGURE 8.

FIGURE 8

The transcription factor ZNF148 regulated PTX3 expression by directly binding to its promoter region. (A, B) Levels of PTX3 in t‐DCs transfected with si‐NC, si‐ZNF148, Vector, or ZNF148‐overexpression plasmids by qRT‐PCR and western blot. (C) ZNF148 binding site in PTX3 promoter region predicted by UCSC. (D) ChIP‐qPCR assay indicating binding of ZNF148 with PTX3 promoter. (E) Oligonucleotide sequences 1 to 9 represent the predicted ZNF148 binding sites by JASPAR. (F) Dual‐luciferase reporter assay indicating binding of PTX3 with ZNF148. (G) Quantitative RT‐PCR on ZNF148 and PTX3 expression in SU3 cells‐derived exosomes. (H) Western blot on ZNF148 and PTX3 expression in SU3 cells‐derived exosomes. (I) Quantitative RT‐PCR on ZNF148 and PTX3 expression in TAAs. (J) Western blot on ZNF148 and PTX3 expression in TAAs. Data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.