Figure 3.

Protein Profiling in Tissue Lysates From the Explanted Native Valves (AS and Normal) and eBVs

(A) Two-dimensional gel electrophoresis of tissue lysates from native and bioprosthetic valves. The left panels are the enlarged figures of the areas surrounded by black broken lines in the right panels. (B) Western blot analyses for fibrinogen, plasminogen (PLG), apolipoprotein E (APOE), and anti–serum amyloid P component (APCS) in tissue lysates from the explanted native and bioprosthetic valves. (C) Statistical quantifications of the western blot results are presented. Analysis of variance, followed by Tukey’s multiple-comparisons test (all comparisons), was used for statistical analyses of the fibrinogen, APCS, and APOE levels. The Kruskal-Wallis test (all comparisons) was used for statistical analyses of PLG levels. ∗P < 0.05 and ∗∗∗P < 0.001. FGB = fibrinogen β-chain; other abbreviations as in Figure 1.