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Activation of the HIS3 reporter by EBNA1 and EBP2 in the two-hybrid system. Tenfold serial dilutions of log-phase cultures of Y190 strains expressing SNF1, EBNA1 or lamin as GAL4 DNA binding domain fusions and SNF4, EBP2, or nothing (pACT2) fused to the GAL4 activation domain were plated on SC-Trp,Leu (left panel) or SC-Trp,Leu,His plus 50 mM AT (right panel). The SNF1/SNF4 culture is a positive control for the two-hybrid interaction.
