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Long-term plasmid maintenance ability of EBNA1 mutants. C33A cells were transfected with a plasmid containing oriP and expressing EBNA1, EBNAΔ325–376 (duplicate samples are shown), EBNAΔ41–376 (duplicate samples are shown), EBNAΔ367–376, EBNAΔ356–362, or no EBNA1 (pc3oriP) and maintained under selection for 14 days. Plasmid DNA from 5 × 106 cells was collected, digested with XhoI and DpnI, and analyzed by Southern blotting. A 100-pg linearized pc3oriPE marker is also shown.
