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. 2023 May 24;14(4):1789–1801. doi: 10.1002/jcsm.13257

Figure 2.

Figure 2

Exercise improves muscle quality via AdipoR1 in mice. (A) Exercise paradigm in mice. Mice at 16 months of age in exercised groups were performed voluntary wheel running for 4 months. (B) The protein levels of AdipoR1 in skeletal muscle were upregulated by exercise. The protein levels of AdipoR1 and AdipoR2 were measured by western blotting (left panel). Quantification of the ratio of either AdipoR1 or AdipoR2 to GAPDH (right panel). (C) Knockdown of Adipor1 inhibited the activation of AMPK induced by exercise. The phosphorylation of AMPK (Thr172) was measured by western blotting (left panel). Quantification of the ratio of p‐AMPK to AMPK (right panel). (D) Muscle mass index of gastrocnemius muscles (n = 5 per group). (E) Muscle weight in gastrocnemius muscles of mice (n = 5 per group). (F) Myonuclei number in gastrocnemius muscles of mice (n = 5 per group). *P < 0.05, ** P < 0.01 and *** P < 0.001. Ad, AdipoR1; NC, negative control; ns, not significant.