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. 2023 May 24;14(4):1789–1801. doi: 10.1002/jcsm.13257

Figure 4.

Figure 4

The AdipoR1–FOXO3a signalling induces autophagy in skeletal muscle of mice after exercise. (A, B) Exercise significantly increased the levels of the LC3B‐II and reduced the levels of p62 in skeletal muscle of exercised mice. The blot is typical of three independent experiments (A). Quantification of the ratio of LC3B‐II to β‐actin (B). Quantification of the ratio of p62 to β‐actin (B). (C, D) Knockdown of either AdipoR1 or FoxO3a by shRNA reduced the levels of the LC3B‐II in skeletal muscle of exercised mice. The blot is typical of three independent experiments (C). Quantification of the ratio of LC3B‐II to β‐tubulin (D). (E, F) Exercise upregulated the protein expression of BNIP3 in the skeletal muscle of mice. Knockdown of either AdipoR1 or FoxO3a by shRNA reduced the levels of the BNIP3 in the skeletal muscle of exercised mice. Representative images of immunohistochemical staining for BNIP3 (E). Scale bars: 50 μm. Quantification of BNIP3 expression in gastrocnemius muscles of mice (F). *P < 0.05, ** P < 0.01 and *** P < 0.001. Ad, AdipoR1; Fo, FoxO3a; NC, negative control; ns, not significant.