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. 2023 May 18;14(4):1802–1814. doi: 10.1002/jcsm.13258

Figure 7.

Figure 7

β‐Alanine and Lewis lung carcinoma cells (LLC) conditioned media (CM) treatment of differentiated skeletal muscle myoblasts (myotubes). Myotubes were treated with LLC CM for 72 h. (A) Cell lysate was analysed for (B) CARNS, TauT, and CNDP2 expression. Data are mean ± SD, n = 5 samples in each group, *P < 0.02 versus non‐treated cells. (C) Levels of carnosine detected by LC–MS/MS in the β‐alanine treated (1 and 5 mM; 12 and 24 h treatment), C2C12 cells. Data are presented as mean ± SD, # P < 0.04 versus non‐treated cells (NT). (D) Levels of the ubiquitinated proteins in myotubes either pretreated with β‐alanine (1 mM) for 12 h followed by LLC CM treatment for 72 h, or post β‐alanine (1 mM) and LLC CM treatments. (E) Data are presented as mean ± SD, n = 5 samples in each group, ^P < 0.05 versus controls, $ P < 0.05 versus LLC CM treated cells.