Figure 2.

Catalytic activity of 25 μM AibH1H2 under different metalation conditions. (A) GC-MS chromatograms filtered at m/z = 218 (Figures S2 and S3) of representative catalytic activity assays of AibH1H2 under different growth conditions with no additional metal added during the activity assay. (B) Catalytic activity of ^FeAibH1H2 and ^MnAibH1H2 (Table 1, entries 1 and 3, respectively) with one additional equivalent of metal or an equal volume of water (denoted with “–”), added to the reaction mixture. (C) Time-dependent generation of d-MeSer by ^Mn*AibH1H2 (black traces, Table 1 entry 4) and ^Fe*AibH1H2 (red traces, Table 1 entry 5) with one additional equivalent of Mn (dashed trace) or Fe (solid trace) added to the reaction mixture.