TABLE 1.
Primers for TMV cDNA synthesis
| Primer | Sequence (5′ to 3′) |
|---|---|
| Reverse primer | CGCGCG CTGCAG CTCGAG ACAACTAGAGCCATCTATAA(4897) |
| PstI XhoI | |
| Primer 1B | GCGCGC GGTACC CCATGG CATACACACAGACAGC(88) |
| KpnI NcoI | |
| Primer H | CGCGCG CTGCAG CTCGAG CCAGGTATTAACCCTGGTGAC(966) |
| PstI XhoI | |
| Primer F | GCGCGC GGTACC CCATGG ATATGCAGTTTTACTATG(3510) |
| KpnI NcoI | |
| Primer A | CGCGCG CTGCAG CTCGAG ACAACTAGAGCCATCTATGAA(4895) |
| PstI XhoI | |
| Primer 3′(+)F | CGC AAGCTT TAATACGACTCACTATA GAGAGCTCTTCTGGTTTGGT(6166) |
| HindIII T7 promoter | |
| Primer 3′(+)R | CGC GGATCC CATATG GGCCCCTACCGGGGGTAACGG(6373) |
| BamHI NdeI |