Table 1.

Culture conditions for different tumor organoids

		Colorectal Cancer		Gastric Cancer	Breast Cancer	Pancreatic Cancer		Cholangiocarcinoma	Lung Cancer	Hepatocellular Cancer	Bladder Cancer	Esophagus Cancer	Thyroid Cancer	Prostate Cancer	Cervical Cancer	Ovarian Cancer	Head and Neck Cancer
Medium	Advance DMEM/F12	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√
	HEPES 10 mM	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√
	Glutamax 2 mM	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√
	Antibiotics/antimycotics (1%)	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√	√
Cytokines	rhR-Spondin-1 (ng/ml)	500	250	1000	250				500			500	250	125	250		100
	rhNoggin (ng/ml)				100	1000	100		100			100	25	100	100		40
	rmNoggin (ng/ml)	100	100	100
	rhFGF basic (ng/ml)										12.5		20	1		50	5
	rhFGF-7 (ng/ml)				5				25		25				25
	rhFGF-10 (ng/ml)			50	20	100			100	100	100	100		10	100		10
	rhIGF (ng/ml)															100
	rhEGF (ng/ml)	50			5	50				50	10	50	20	50	50		50
	rmEGF (ng/ml)		50	50			50	50
	rhWnt-3a (ng/ml)	50	50	25		50	25					50	50			20
	rhVEGF121 (ng/ml)												10
	rhNeuregulin 1 (nM)				5
	rhHGF (ng/ml)									25
Additive	N-2	1				1		1		1		1
	N-21	1	1	1	1	1	1	1	1	1	1		0.5	1	1	1	1
Small Molecular Compounds	Wnt-C59						100
	Y-27,632 (µM)				5			10	5	10	10		10	10	10	10
	A83-01 (nM)	500	500	500	500	500	500	5000	500	5	5000	500	5000	500	500	500
	Dihydrotestosterone (DHT) (nM)													0.1/1
	Primocin													1:100v/v
	Gastrin I (Human) (nM)	10	10	10		10		10		10						10
	Forskolin (µM)							10		10					10		1
	Dexamethasone (nM)	10	10							3
	SB 202,190				500 nM	10 mM	10 µM							10 µM	1 µM
	Prostaglandin E2 (µM)								500								1
	Beta-Estradiol (nM)														100
	Wnt Surrogate (nM)														0.5
	CHIR 99,021 (µM)														0.3		3
	Nicotinamide (mM)	10			5	10		10	5	10	10	10	0.1	10	2.5
	N-Acetylcysteine (mM)	1.25	1	1	1.25	1	1	1	1.25	1.25	1.25			1.25	1.25	1	1.25
References		[79]	[82]	[136]	[51]	[50]	[170]	[102]	[171]	[15]	[172]	[149]	[173]	[10]	[174]	[175]	[176]