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[Preprint]. 2023 Jul 26:2023.06.20.545631. [Version 2] doi: 10.1101/2023.06.20.545631

Figure 3. Adult Kcnk16 L114P mice show disrupted islet hormone secretion and islet composition.

Figure 3.

A. and B. Plasma insulin levels in male (A) and female (B) WT and Kcnk16 L114P(L/P) mice following a 4-hour fast at the indicated time points before and after a 2mg/g glucose injection. c. Glucose (%) change in response to an i.p. human insulin injection (0.75 UI/kg body weight) was measured as an indicator of insulin sensitivity in WT and Kcnk16 L114P male mice after a 4-hour fast. D. and E. In-vitro insulin secretion (N=5 mice/genotype) and glucagon secretion (N=3 mice/genotype) from male mice at the specified glucose concentrations F. Representative immunostaining images of pancreas sections from WT and Kcnk16 L114P(L/P) male mice (N=3/genotype) stained against insulin (INS; green), glucagon (GCG; red), somatostatin (SST; magenta), and Hoechst (blue); scale bar = 100 μm. G-J. Average islet area and area of hormone staining/ islet for β-cells (INS), α-cells (GCG), and δ-cells (SST). Data are presented as mean±SEM. Data were analyzed using student’s t-test or two-way ANOVA as appropriate.