Table 2.

Contacts between activation loop residues and substrate

Kinase (Family Gene Spec.)	PDB	Len	DFG	4	5	6		15	14	13	12	11	10	9	8	7	6	5	4	APE
AGC_AKT1_HUMAN	4ekkA	10		X						X	X	X	X	X	X	X	X	X	X
AGC_AKT2_HUMAN	1o6lA	10		X							X	X	X	X	X	X	X	X	X
AGC_PRKACA_MOUSE	1l3rE	20	X	X							X	X	X	X	X	X	X	X	X
AGC_PRKACA_MOUSE	2qvsE	310		X						X	X	X	X	X	X	X	X	X	X
AGC_PRKACA_MOUSE	3idbA	161		X						X	X	X	X	X	X	X	X	X	X
AGC_PRKACA_MOUSE	7e0zA	12		X								X	X	X	X	X	X	X	X
AGC_PRKCI_MOUSE	4dc2A	28		X							X	X	X	X	X	X	X	X	X
AGC_PRKCI_HUMAN	5lihA	16	X	X	X						X	X	X	X	X	X	X
CAMK_CAMK2A_HUMAN	7uirA	19	X	X								X	X	X	X	X	X	X
CAMK_CAMK2D_HUMAN	2welB	327		X								X	X	X	X	X	X	X
CAMK_CAMKII_CAEEL	3kk9B	282	X	X								X	X	X	X	X	X	X
CAMK_PHKG1_RABIT	2phkA	7	X	X							X	X	X	X	X	X	X	X
CAMK_PIM1_HUMAN	2bzkB	15		X								X	X	X	X		X
CK1_CSNK1D_HUMAN	6ru7A	15	X	X	X	X				X		X	X	X	X	X
CMGC_CDK2_HUMAN	1qmzA	7	X	X		X				X		X	X	X	X	X	X		X
CMGC_CDK2_HUMAN	3qhrA	10	X	X						X		X	X	X	X	X	X		X
CMGC_CLK2_HUMAN	3nr9A	368										X	X	X	X	X	X	X	X
CMGC_DYRK1A_HUMAN	2wo6A	8		X						X		X	X	X	X	X	X	X	X
OTHER_CDC7_HUMAN	6ya7A	15	X	X		X				X		X	X	X	X	X		X	X
OTHER_HASPIN_HUMAN	4oucA	12		X
STE_PAK1_HUMAN	4zy4A	329	X	X										X	X		X	X
STE_PAK4_HUMAN	2q0nA	11		X						X	X	X	X	X	X	X	X	X	X
TKL_BAK1_ARATH	3tl8A	117		X								X	X	X	X	X
TKL_IRAK4_HUMAN	4u97A			X						X	X	X	X	X	X	X	X		X
TYR_ABL1_HUMAN	2g2iA	13								X		X	X	X	X	X		X
TYR_CSF1R_HUMAN	3lcdB	329	X	X							X	X	X	X	X	X	X	X	X
TYR_EGFR_HUMAN	5czhA	9	X								X	X	X	X	X	X	X	X
TYR_EPHA2_HUMAN	4pdoA	299										X	X	X	X	X	X	X
TYR_EPHA3_HUMAN	3fxxA	10		X									X	X	X	X	X	X
TYR_FES_HUMAN	3cblA	6		X						X	X	X	X	X	X	X		X
TYR_FGFR1_HUMAN	3gqiA	326		X							X	X	X	X	X	X		X
TYR_FGFR2_HUMAN	2pvfA	15	X	X									X	X	X	X		X
TYR_FGFR2_HUMAN	3clyB	334				X				X	X	X	X	X	X	X	X	X
TYR_FGFR3_HUMAN	4k33B	325	X	X						X	X	X	X	X	X	X		X
TYR_IGF1R_HUMAN	1k3aA	14		X				X	X	X	X	X	X	X	X	X		X	X
TYR_IGF1R_HUMAN	3lvpB	336		X	X	X							X	X	X	X		X
TYR_INSR_HUMAN	1ir3A	18	X	X						X	X	X	X	X	X	X	X
TYR_INSR_HUMAN	3bu5A	15	X	X				X	X	X	X	X	X	X	X	X	X	X	X
TYR_KIT_HUMAN	1pkgB	320	X	X								X	X	X	X		X	X
TYR_SYK_HUMAN	5c27A	5	X	X							X	X	X	X	X	X	X

For each kinase, contacts between substrate and activation loops residues are marked with an “X”. A contact with any residue of the DFG motif is listed under “DFG.” Residues 4, 5, and 6 of the activation loop are in the adjacent columns. Contacts for the C-terminal region of the activation loop are to the right of the shaded area, starting with residues 15, 14, 13, …, from the end of the activation loop which typically has the sequence motif “APE” (often “SPE” or “PPE”).