FIG. 4.

Kinetics of DV replication in primary human MO/Mφ. Infection experiments on 7-day-old (A) and 6-day-old (B) MO/Mφ cultures were performed with three comparison groups (DV alone, LPS-DV, and DV-LPS) as described in the legend to Fig. 1. For LPS-DV, LPS at a concentration of 6 μg/ml was incubated with the cells for 22 h prior to infection and for DV-LPS, LPS was added to the cultures immediately after viral adsorption. All cultures were infected with DV at an MOI of 3 PFU per cell and then incubated to follow the short-term (A) and long-term (B) kinetics of extracellular and intracellular infectious virus production. For each time point, three separate wells were prepared and analyzed, and the results are presented as the mean ± the SE.