FIG. 6.

Blockade of LPS-induced suppression of DV infection by two anti-CD14 MAbs, MY4 and MoS39. One-week-old MO/Mφ were infected with DV at an MOI of 3 PFU per cell without any treatment (a) or were treated with LPS (5 μg/ml) for 20 h prior to DV infection (b). In addition, MO/Mφ were preincubated with 10 or 20 μg of MY4 per ml (c and d, respectively) or with 20 or 40 μg of MoS39 per ml (e and f, respectively) for 2 h before the addition of LPS (i.e., 22 h prior to DV infection). Some cultures were incubated with 10 μg of MY4 per ml for 2 h (g) or 20 μg of MoS39 per ml for 22 h (h) prior to DV infection, without a subsequent LPS treatment. In some cultures, MY4 was added after 2 h of viral adsorption (i). Each experimental point represents the mean ± the SE of results obtained from three separate wells.