Table 1: Summary of piRNA single mutant and mnk double mutant phenotypes.

Perinuclear nuage localization of Vasa, Aub, ago3 and Armi proteins were evaluated in various piRNA mutants and designated as more localized than wild type (+), wildtype like localization (WT), less localized than wildtype (−), not localized at all (O) and not applicable (NA). Long and small RNA sequencing were performed on various piRNA mutants and changes in global levels relative to w¹ controls were evaluated. Up relative to controls is designated by +, down relative to controls is designated by -, and comparable levels is designated by WT.

		Nuage Localization of:				piRNA			Long RNA
	Genotype	Vasa	Aub	Ago3	Armi	antisense TE	z-score	Phasing	TE
RDC	rhi KG/02	−	O	−	−	− −	− −	WT	++
	mnk, rhi KG/02	−	O	−	−	− −	− −	WT	++
TREX Export Machinery	uap56 sz16/28	−	−	−	O	−	−	WT	++
	mnk, uap56 sz15/28	WT	WT	WT	O	−	−	WT	+
	thoc7 d/Df	−	O	WT	−	−	WT	WT	+
	mnk; thoc7 d/Df	WT	O		−	−	WT	WT	+
Ping-Pong Machinery	vas RG/PH	NA	O	WT	WT	−	−	WT	++
	mnk, vas RG/PH	NA	O	WT	WT	−	−	WT	++
	aub HN/QC	WT	NA	WT	WT	− −	− −	WT	+
	mnk, aub HN/QC	WT	NA	WT	WT	− −	− −	WT	+
	ago3 t2/t3	WT	O	NA	−	− −	− −	WT	++
	mnk; ago3 t2/t3	WT	−	NA	−	− −	− −	WT	++
Transcrptional Silencing	armi 1/72.1	WT		WT	NA	− −	− −	WT	++
	mnk; armi 1/72.1	WT		WT	NA	− −	− −	WT	++
	mnk, piwi 02/ΔNLS	ovaries were unrecoverable