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[Preprint]. 2024 Jan 13:2023.07.25.550548. Originally published 2023 Jul 26. [Version 2] doi: 10.1101/2023.07.25.550548

Figure 1. Calcineurin regulates the onset and reversion of transgene silencing.

Figure 1.

(A) A cartoon depicting the URA5 transgene array in the reporter strain JF289. (B) A schematic showing the workflow to determine the mitotic-induced silencing (MIS) frequency with the reporter strain. (C) Plate photos showing the difference in colony morphology for the wild-type JF289, cna1Δ, cnb1Δ, and JF289 plated on FK506 containing media when an approximately equal number of colonies were obtained in each case after 10 days of incubation. (D) A graph showing the MIS frequency of calcineurin mutants as compared to the JF289 strain. P-value (**) < 0.01. Error bars represent the standard error of the mean (SEM). (E) Images of petri-dishes showing the growth of colonies on media lacking uracil (SD-URA) directly from 5-FOA-containing media during MIS assays. The numbers below represent the average percent of colonies growing (Mean ± SEM) from three independent experiments.