Table 2.
Condition | siRNA length | Percent of reads in siRNA population | ||||
---|---|---|---|---|---|---|
Wild-type | cna1Δ | cnb1Δ | gwo1Δ | puf4Δ | ||
YPD | 21-nt | 7.30 | 4.08 | 3.48 | 8.06 | 5.57 |
22-nt | 15.15 | 8.02 | 6.66 | 15.30 | 10.02 | |
23-nt | 8.58 | 5.40 | 4.55 | 8.02 | 5.34 | |
Total | 31.03 | 17.50 | 14.69 | 31.38 | 20.93 | |
5-FOA-1 | 21-nt | 8.68 | 6.01 | 5.78 | 7.33 | 8.56 |
22-nt | 16.57 | 11.39 | 10.62 | 13.89 | 15.54 | |
23-nt | 8.54 | 6.59 | 6.13 | 6.33 | 7.82 | |
Total | 33.79 | 23.99 | 22.53 | 27.55 | 31.92 | |
5-FOA-2 | 21-nt | 9.69 | 6.52 | 5.94 | 8.68 | 9.65 |
22-nt | 17.85 | 12.47 | 11.19 | 17.14 | 16.88 | |
23-nt | 8.86 | 7.29 | 6.32 | 8.04 | 7.07 | |
Total | 36.40 | 26.28 | 23.45 | 33.86 | 33.60 | |
Mean ± SEM of total siRNA/condition | 33.74±1.55 | 22.59±2.63 | 20.22±2.78 | 30.93±1.84 | 28.81±3.97 | |
Significant; p-value (Compared to wild-type) | Yes; 0.011 | Yes; 0.012 | No; 0.278 | No; 0.199 |