REAGENT or RESOURCE	SOURCE or REFERENCE	IDENTIFIERS
Antibodies
Rabbit polyclonal anti-NPY	BMA Biomedicals	(Cat# T-4070, RRID:AB_518504)
Chicken polyclonal anti-GFP	Abcam	(Cat# ab13970, RRID:AB_300798)
Rabbit polyclonal anti-tyrosine hydroxylase	Millipore	(Cat# ab152, RRID:AB_390204)
Mouse monoclonal anti-tyrosine hydroxylase	Sigma-Aldrich	(Cat# T2928, RRID:AB_477569)
Sheep polyclonal anti-tyrosine hydroxylase	Millipore	(Cat# ab1542, RRID:AB_90755)
Rabbit monoclonal anti-RFP	Abcam	(Cat# ab185921, RRID:AB_2934052)
Rabbit polyclonal anti-cFos	Abcam	(Cat# ab190289; RRID:AB_2737414)
Rabbit monoclonal anti-cFos	Abcam	(Cat# ab222699, RRID:AB_2891049)
Donkey anti-Rabbit IgG Alexa Flour 647	Thermo Fisher Scientific	(Cat# A-31573, RRID:AB_2536183)
Donkey anti-Chicken IgG Alexa Flour 488	Sigma-Aldrich	(Cat# SAB4600031, RRID:AB_2721061)
Donkey anti-Sheep IgG Alexa Flour 546	Thermo Fisher Scientific	(Cat# A-21098, RRID:AB_2535752)
Donkey anti-Sheep IgG Alexa Flour 647	Abcam	(Cat# ab150179, RRID:AB_2884038)
Goat anti-Mouse IgG1 Alexa 647	Thermo Fisher Scientific	(Cat# A-21240, RRID:AB_2535809)
Goat anti-Rabbit IgG Alexa 647	Thermo Fisher Scientific	(Cat# A-21244, RRID:AB_2535812)
Goat anti-Rabbit IgG Alexa 546	Thermo Fisher Scientific	(Cat# A-11035, RRID:AB_2534093)
Chemicals, peptides, and recombinant proteins
Glycogen, RNA grade	Thermo Scientific Fisher	Cat# R0551
Trizol	Thermo Scientific Fisher	Cat# 15596018
Maxima SYBR Green/ROX qPCR Master Mix (2X)	Thermo Scientific Fisher	Cat# K0222
Paraformaldehyde, reagent grade	Sigma-Aldrich	Cat# P6148-500G
Bouin’s solution	Sigma-Aldrich	Cat# HT10132-1L
DAPI	Roche	Cat# 10236276001
ProLong Gold Antifade Mountant	Thermo Scientific Fisher	Cat# P36930
Fluoromount Mounting Media	Sigma-Aldrich	Cat# F4680-25mL
Permount Mounting Media	Fisher Scientific	Cat# SP11500
Hydrogen peroxide solution, 30%in H2O, ACS reagent	Sigma-Aldrich	Cat# 216763
Heparin sodium salt	Sigma-Aldrich	Cat# H3393
Isoflurane	MWI	Cat# 502017
Insulin	Novolin-R	Cat# A10005804
D-(+)-Glucose, ACS reagent	Sigma-Aldrich	Cat# G5767-25G
Histopaque(R)-1077	Sigma-Aldrich	Cat#10771
Histopaque(R)-1119	Sigma-Aldrich	Cat#11191
Collagenase P	Sigma-Aldrich	Cat#11249002001
Critical commercial assays
Superscript IV First Strand Synthesis System	Thermo Fisher Scientific	Cat# 18091050
Ultra-Sensitive Mouse Insulin ELISA Kit	Crystal Chem	Cat# 90080
PicoGreen Kit	Thermo Fisher Scientific	Cat#P7581
Norepinephrine High Sensitive ELISA Kit	Rocky Mountain Diagnostics	Cat# BA E-5200R
Experimental models: Organisms/strains
Mouse: B6.Cg-Npytm1(cre)Zman/J	The Jackson Laboratory	RRID:IMSR_JAX:027851
Mouse: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J	The Jackson Laboratory	RRID:IMSR_JAX:006148
Mouse: DBH-Cre	Quach et. al., 2013
Mouse: B6.Cg-Npytm1.1(flpo)Hze/J	The Jackson Laboratory	RRID:IMSR_JAX:030211
Mouse: B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J	The Jackson Laboratory	RRID:IMSR_JAX:021875
Mouse: NPYfl/fl	(Wee et al., 2019)
Mouse: C57Bl/6J (Strain background)	The Jackson Laboratory	RRID:IMSR_JAX:000664
Oligonucleotides
Npy_F:5’-AGAGATCCAGCCCTGAGACA-3’	This paper	qPCR primers
Npy_R:5’-GATGAGGGTGGAAACTTGGA-3’	This paper	qPCR primers
Th_F:5'-AATCCACCACTTAGAGACCCG	This paper	qPCR primers
Th_R:3'-CTTGGTGACCAGGTGGTGAC	This paper	qPCR primers
18s_F:5'-CGCCGCTAGAGGTGAAATTC	(Ceasrine et al., 2018)	qPCR primers
18s_R:5'-TTGGCAAATGCTTTCGCTC	(Ceasrine et al., 2018)	qPCR primers
Npyfl/fl_F:5'-GCCTCCGTGCCTCTTATCTT-3'	(Wee et al., 2019)	Genotyping primers
Npyfl/fl_R:5'-GATTTTGGAGGCAACAGAGC-3'	(Wee et al., 2019)	Genotyping primers
B6.Cg-Npytm1(cre)Zman/J_F:5'-TCC ATG ATT TGC CTC TTG TG -3'	The Jackson Laboratory	Genotyping primers
B6.Cg-Npytm1(cre)Zman/J_R:5'-ACA CCG GCC TTA TTC CAA G -3'	The Jackson Laboratory	Genotyping primers
B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/_F:5'-AGG GCG AGG AGC TGT TCA-3'	The Jackson Laboratory	Genotyping primers
B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J_R:5'-TGA AGT CGA TGC CCT TCA G -3'	The Jackson Laboratory	Genotyping primers
DBH-Cre_F:5'-CTG CCA GGG ACA TGG CCA GG-3'	(Quach et al., 2013)	Genotyping primers
DBH-Cre_R:5'-GCA CAG TCG AGG CTG ATC AGC-3'	(Quach et al., 2013)	Genotyping primers
B6.Cg-Npytm1.1(flpo)Hze/J_F:5'-TGC TGC TGT TCT CCG TAG C-3'	The Jackson Laboratory	Genotyping primers
B6.Cg-Npytm1.1(flpo)Hze/J_R:5'-ACA CCG GCC TTA TTC CAA G-3'	The Jackson Laboratory	Genotyping primers
B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J_F:5'-CTG TTC CTG TAC GGC ATG G-3'	The Jackson Laboratory	Genotyping primers
B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J_R:5'-GGC ATT AAA GCA GCG TAT CC -3'	The Jackson Laboratory	Genotyping primers
Software and algorithms
Ponemah Software	N/A	https://www.datasci.com/products/software/ponemah
Anilogger Manager software	N/A	https://www.animals-monitoring.fr/wp-content/uploads/2020/06/P120GUI004-Quick.Start_Anilogger.system.EN_pdf
Imaris	Oxford	https://imaris.oxinst.com
ImageJ	N/A	https://ImageJ.nih.gov/ij/
ZEN 2012 (blue edition)	N/A	https://www.zeiss.com/microscopy/int/home.html
GraphPad Prism9	N/A	https://www.graphstats.net/graphpad-prism